2017 ACS Synthetic Biology Issues

Summary

  • Total Articles Analyzed: 242
  • Total Figures in All Articles: 1266
  • Figures Compatible with SBOL Visual: 285 (22.51%)
  • Figures Compatible with SBOL Visual & Fully Compliant: 187 (65.61%)
  • Figures Compatible with SBOL Visual & Following Best Practices: 115 (40.35%)

Individual issues

January

ArticleNo. of SBOL Visual compliant imagesNo. of SBOL Visual images following Best practices
Rapid and Inexpensive Evaluation of Nonstandard Amino Acid Incorporation in Escherichia coli11
Designing Synthetic Flexible Gene Regulation Networks Using RNA Devices in Cyanobacteria11
Short Synthetic Terminators for Assembly of Transcription Units in Vitro and Stable Chromosomal Integration in Yeast S. cerevisiae52
Improving Metabolic Pathway Efficiency by Statistical Model-Based Multivariate Regulatory Metabolic Engineering33

February

ArticleNo. of SBOL Visual compliant imagesNo. of SBOL Visual images following Best practices
Discovery of a Phosphonoacetic Acid Derived Natural Product by Pathway Refactoring10
Direct Combinatorial Pathway Optimization10
Artificial Symmetry-Breaking for Morphogenetic Engineering Bacterial Colonies54
Synthetic Biogenesis of Bacterial Amyloid Nanomaterials with Tunable Inorganic–Organic Interfaces and Electrical Conductivity10
Resource Sharing Controls Gene Expression Bursting10
Characterization of Endogenous and Reduced Promoters for Oxygen-Limited Processes Using Escherichia coli11
Reproducibility of High-Throughput Plate-Reader Experiments in Synthetic Biology10

March

ArticleNo. of SBOL Visual compliant imagesNo. of SBOL Visual images following Best practices
Standardized Markerless Gene Integration for Pathway Engineering in Yarrowia lipolytica10
Introduction of Premature Stop Codons as an Evolutionary Strategy To Rescue Signaling Network Function20
Synthetic Core Promoters as Universal Parts for Fine-Tuning Expression in Different Yeast Species10
Reprogramming the Transcriptional Response to Hypoxia with a Chromosomally Encoded Cyclic Peptide HIF-1 Inhibitor20
A Novel Approach for Gene Expression Optimization through Native Promoter and 5′ UTR Combinations Based on RNA-seq, Ribo-seq, and TSS-seq of Streptomyces coelicolor10

April

ArticleNo. of SBOL Visual compliant imagesNo. of SBOL Visual images following Best practices
Engineering a Thermostable Keto Acid Decarboxylase Using Directed Evolution and Computationally Directed Protein Design10
Quantitative Tracking of Combinatorially Engineered Populations with Multiplexed Binary Assemblies10
Controlling Multicycle Replication of Live-Attenuated HIV-1 Using an Unnatural Genetic Switch22

May

ArticleNo. of SBOL Visual compliant imagesNo. of SBOL Visual images following Best practices
AGOS: A Plug-and-Play Method for the Assembly of Artificial Gene Operons into Functional Biosynthetic Gene Clusters33
Efficient Genome Editing of a Facultative Thermophile Using Mesophilic spCas921
Mechanistic Modeling of Genetic Circuits for ArsR Arsenic Regulation22
An Engineered Survival-Selection Assay for Extracellular Protein Expression Uncovers Hypersecretory Phenotypes in Escherichia coli10

June

ArticleNo. of SBOL Visual compliant imagesNo. of SBOL Visual images following Best practices
Engineering Iron Responses in Mammalian Cells by Signal-Induced Protein Proximity30
Recursive DNA Assembly Using Protected Oligonucleotide Duplex Assisted Cloning (PODAC)22
Extracellular Self-Assembly of Functional and Tunable Protein Conjugates from Bacillus subtilis11
Tailoring Escherichia coli for the l-Rhamnose PBAD Promoter-Based Production of Membrane and Secretory Proteins30
Heterologous Biosynthesis of Spinosad: An Omics-Guided Large Polyketide Synthase Gene Cluster Reconstitution in Streptomyces44
A Modular Toolkit for Generating Pichia pastoris Secretion Libraries55
Development of a Biosensor Concept to Detect the Production of Cluster-Specific Secondary Metabolites21
A Scalable Epitope Tagging Approach for High Throughput ChIP-Seq Analysis11
Formation of Nitrogenase NifDK Tetramers in the Mitochondria of Saccharomyces cerevisiae11
Obtaining a Panel of Cascade Promoter-5′-UTR Complexes in Escherichia coli10
The Impact of DNA Topology and Guide Length on Target Selection by a Cytosine-Specific Cas922

July

ArticleNo. of SBOL Visual compliant imagesNo. of SBOL Visual images following Best practices
DNAplotlib: Programmable Visualization of Genetic Designs and Associated Data55
SBOLDesigner 2: An Intuitive Tool for Structural Genetic Design33
A Validator and Converter for the Synthetic Biology Open Language22
Simulation Approach for Timing Analysis of Genetic Logic Circuits22
Automated Design Framework for Synthetic Biology Exploiting Pareto Optimality11
sbml-diff: A Tool for Visually Comparing SBML Models in Synthetic Biology11
Improvement of Squalene Production from CO2 in Synechococcus elongatus PCC 7942 by Metabolic Engineering and Scalable Production in a Photobioreactor20
Measurement of In Vivo Protein Binding Affinities in a Signaling Network with Mass Spectrometry21
Genome Engineering of Virulent Lactococcal Phages Using CRISPR-Cas930
Deconvolution of Gene Expression Noise into Spatial Dynamics of Transcription Factor–Promoter Interplay44
EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors66
Construction and Rescue of a Functional Synthetic Baculovirus22
Repurposing a Two-Component System-Based Biosensor for the Killing of Vibrio cholerae22

August

ArticleNo. of SBOL Visual compliant imagesNo. of SBOL Visual images following Best practices
Amplifying Riboswitch Signal Output Using Cellular Wiring22
Rational Design of an Ultrasensitive Quorum-Sensing Switch11
Genome-Wide Abolishment of Mobile Genetic Elements Using Genome Shuffling and CRISPR/Cas-Assisted MAGE Allows the Efficient Stabilization of a Bacterial Chassis11
A New Improved and Extended Version of the Multicell Bacterial Simulator gro11
Synthetic Protein Scaffolds for Biosynthetic Pathway Colocalization on Lipid Droplet Membranes22
Programming Post-Translational Control over the Metabolic Labeling of Cellular Proteins with a Noncanonical Amino Acid11
Sort-Seq Approach to Engineering a Formaldehyde-Inducible Promoter for Dynamically Regulated Escherichia coli Growth on Methanol11

September

ArticleNo. of SBOL Visual compliant imagesNo. of SBOL Visual images following Best practices
Engineering a Native Inducible Expression System in Shewanella oneidensis to Control Extracellular Electron Transfer22
Multiplex Genome Editing by Natural Transformation (MuGENT) for Synthetic Biology in Vibrio natriegens22
Rapid Generation of Universal Synthetic Promoters for Controlled Gene Expression in Both Gas-Fermenting and Saccharolytic Clostridium Species21
Mini Photobioreactors for in Vivo Real-Time Characterization and Evolutionary Tuning of Bacterial Optogenetic Circuit10

October

ArticleNo. of SBOL Visual compliant imagesNo. of SBOL Visual images following Best practices
Developing a Genetically Encoded, Cross-Species Biosensor for Detecting Ammonium and Regulating Biosynthesis of Cyanophycin22
Rapid and Programmable Protein Mutagenesis Using Plasmid Recombineering22
A Synthetic Circuit for Mercury Bioremediation Using Self-Assembling Functional Amyloids10
Fundamental Design Principles for Transcription-Factor-Based Metabolite Biosensors30
Evidence for Improved Encapsulated Pathway Behavior in a Bacterial Microcompartment through Shell Protein Engineering11
A Versatile Toolbox for the Control of Protein Levels Using Nε-Acetyl-l-lysine Dependent Amber Suppression40
Engineered Control of Genetic Variability Reveals Interplay among Quorum Sensing, Feedback Regulation, and Biochemical Noise11
Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins44
Development of Chemical and Metabolite Sensors for Rhodococcus opacus PD63011

November

ArticleNo. of SBOL Visual compliant imagesNo. of SBOL Visual images following Best practices
The Timing of Transcriptional Regulation in Synthetic Gene Circuits30
A Tug-of-War Mechanism for Pattern Formation in a Genetic Network20
A Small-Molecule Inducible Synthetic Circuit for Control of the SOS Gene Network without DNA Damage11
Orthogonal Ribosome Biofirewall50
Genetic Design via Combinatorial Constraint Specification22
Systematic Approaches to Efficiently Produce 2,3-Butanediol in a Marine Cyanobacterium11
Construction of Recombinant Pdu Metabolosome Shells for Small Molecule Production in Corynebacterium glutamicum22
Dynamic Blue Light-Inducible T7 RNA Polymerases (Opto-T7RNAPs) for Precise Spatiotemporal Gene Expression Control20

December

ArticleNo. of SBOL Visual compliant imagesNo. of SBOL Visual images following Best practices
Production of Functional Anti-Ebola Antibodies in Pichia pastoris10
Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in E. coli through Transient Inhibition of Host RecA Activity10
Leaf LIMS: A Flexible Laboratory Information Management System with a Synthetic Biology Focus11
Engineering Isoprene Synthase Expression and Activity in Cyanobacteria11
Genetic Constructor: An Online DNA Design Platform20