2022 ACS Synthetic Biology Issues
Summary
- Total Articles Analyzed: 393
- Total Figures in All Articles: 2068
- Figures Compatible with SBOL Visual: 595 (28.77%)
- Figures Compatible with SBOL Visual & Fully Compliant: 487 (81.85%)
- Figures Compatible with SBOL Visual & Following Best Practices: 245 (41.18%)
Individual issues
January
| Article | No. of SBOL Visual compliant images | No. of SBOL Visual images following Best practices | |
|---|---|---|---|
| Synthetic Biology in Chimeric Antigen Receptor T (CAR T) Cell Engineering | 3 | 3 | ⭐ |
| Advances in Approaches to Study Chromatin-Mediated Epigenetic Memory | 1 | 0 | |
| Repurposing the Homing Endonuclease I-SceI for Positive Selection and Development of Gene-Editing Technologies | 1 | 1 | ⭐ |
| Development and Application of a New Arabinose-Inducible Vector in High-Attachment Strain Stenotrophomonas AGS-1 from Aerobic Granular Sludge | 2 | 0 | |
| CRISPR-Cas9-mediated Large Cluster Deletion and Multiplex Genome Editing in Paenibacillus polymyxa | 2 | 2 | ⭐ |
| Reprogramming the Biosynthesis of Precursor Peptide to Create a Selenazole-Containing Nosiheptide Analogue | 3 | 3 | ⭐ |
| TALE.Sense: A Versatile DNA Sensor Platform for Live Mammalian Cells | 4 | 0 | |
| Engineering a Controllable Targeted Protein Degradation System and a Derived OR-GATE-Type Inducible Gene Expression System in Synechococcus elongatus PCC 7942 | 4 | 4 | ⭐ |
| Synergistic Metabolism of Glucose and Formate Increases the Yield of Short-Chain Organic Acids in Escherichia coli | 1 | 0 | |
| Multiloci Manipulation of Baculovirus Genome Reveals the Pivotal Role of Homologous Regions in Viral DNA Replication, Progeny Production, and Enhancing Transcription | 1 | 0 | |
| Incorporation of Functional Rubisco Activases into Engineered Carboxysomes to Enhance Carbon Fixation | 1 | 1 | ⭐ |
| Artificial Consortium of Three E. coli BL21 Strains with Synergistic Functional Modules for Complete Phenanthrene Degradation | 2 | 0 | |
| Interaction of Bare dSpCas9, Scaffold gRNA, and Type II Anti-CRISPR Proteins Highly Favors the Control of Gene Expression in the Yeast S. cerevisiae | 1 | 0 | |
| Episome-Based Gene Expression Modulation Platform in the Model Diatom Phaeodactylum tricornutum | 4 | 1 | |
| Cell-Free Gene Expression Dynamics in Synthetic Cell Populations | 2 | 2 | ⭐ |
| Mapping of Nonhomologous End Joining-Mediated Integration Facilitates Genome-Scale Trackable Mutagenesis in Yarrowia lipolytica | 3 | 3 | ⭐ |
| Antibiotic-Efficient Genetic Cassette for the TEM-1 β-Lactamase That Improves Plasmid Performance | 2 | 2 | ⭐ |
| Evolving a Generalist Biosensor for Bicyclic Monoterpenes | 3 | 3 | ⭐ |
| Engineering a Dynamic Controllable Infectivity Switch in Bacteriophage T7 | 1 | 0 | |
| Design and Characterization of an Optogenetic System in Pichia pastoris | 1 | 0 | |
| CRISPR-Based Construction of a BL21 (DE3)-Derived Variant Strain Library to Rapidly Improve Recombinant Protein Production | 4 | 4 | ⭐ |
| A Self-Sustained System Spanning the Primary and Secondary Metabolism Stages to Boost the Productivity of Streptomyces | 8 | 7 | |
| Bio-SCAN: A CRISPR/dCas9-Based Lateral Flow Assay for Rapid, Specific, and Sensitive Detection of SARS-CoV-2 | 1 | 0 | |
| Cheating the Cheater: Suppressing False-Positive Enrichment during Biosensor-Guided Biocatalyst Engineering | 3 | 3 | ⭐ |
| CS-Cells: A CRISPR-Cas12 DNA Device to Generate Chromosome-Shredded Cells for Efficient and Safe Molecular Biomanufacturing | 3 | 1 | |
| Engineering a Synthetic Pathway for Tyrosol Synthesis in Escherichia coli | 2 | 0 | |
| CRISPR/Cas-Based Genome Editing for Human Gut Commensal Bacteroides Species | 2 | 2 | ⭐ |
| Engineering of the Ligand Specificity of Transcriptional Regulator XylS by Deep Mutational Scanning | 1 | 1 | ⭐ |
| Building a Versatile Protein Production Platform Using Engineered Trichoderma reesei | 3 | 0 | |
| A Tunable and Expandable Transactivation System in Probiotic Yeast Saccharomyces boulardii | 2 | 2 | ⭐ |
February
| Article | No. of SBOL Visual compliant images | No. of SBOL Visual images following Best practices | |
|---|---|---|---|
| Combating Infectious Diseases with Synthetic Biology | 2 | 0 | |
| Construction of Synthetic Microbial Ecosystems and the Regulation of Population Proportion | 2 | 0 | |
| Building an RNA-Based Toggle Switch Using Inhibitory RNA Aptamers | 4 | 0 | |
| Regulatory Part Engineering for High-Yield Protein Synthesis in an All-Streptomyces-Based Cell-Free Expression System | 4 | 4 | ⭐ |
| EMMA-CAD: Design Automation for Synthetic Mammalian Constructs | 4 | 4 | ⭐ |
| Automation and Expansion of EMMA Assembly for Fast-Tracking Mammalian System Engineering | 1 | 1 | ⭐ |
| Synthetic Biology Toolkit for Marker-Less Integration of Multigene Pathways into Pichia pastoris via CRISPR/Cas9 | 2 | 2 | ⭐ |
| One-Step In Vivo Assembly of Multiple DNA Fragments and Genomic Integration in Komagataella phaffii | 3 | 0 | |
| Conversion of the OmpF Porin into a Device to Gather Amyloids on the E. coli Outer Membrane | 1 | 0 | |
| Reduction of the Bacterial Genome by Transposon-Mediated Random Deletion | 1 | 0 | |
| Engineering a Xylose-Utilizing Synechococcus elongatus UTEX 2973 Chassis for 3-Hydroxypropionic Acid Biosynthesis under Photomixotrophic Conditions | 2 | 2 | ⭐ |
| Codon-Restrained Method for Both Eliminating and Creating Intragenic Bacterial Promoters | 3 | 3 | ⭐ |
| Differentially Optimized Cell-Free Buffer Enables Robust Expression from Unprotected Linear DNA in Exonuclease-Deficient Extracts | 5 | 5 | ⭐ |
| A Simple Biosensor-Based Assay for Quantitative Autoinducer-2 Analysis | 2 | 0 | |
| Enhancement of l-Pipecolic Acid Production by Dynamic Control of Substrates and Multiple Copies of the pipA Gene in the Escherichia coli Genome | 1 | 1 | ⭐ |
| Secretory Production of Tocotrienols in Saccharomyces cerevisiae | 1 | 0 | |
| Electrogenetic Signal Transmission and Propagation in Coculture to Guide Production of a Small Molecule, Tyrosine | 1 | 0 | |
| Engineered Mutants of a Marine Photosynthetic Purple Nonsulfur Bacterium with Increased Volumetric Productivity of Polyhydroxyalkanoate Bioplastics | 1 | 0 | |
| Highly Reversible Tunable Thermal-Repressible Split-T7 RNA Polymerases (Thermal-T7RNAPs) for Dynamic Gene Regulation | 3 | 0 | |
| Establishment of Green- and Red-Fluorescent Reporter Proteins Based on the Fluorescence-Activating and Absorption-Shifting Tag for Use in Acetogenic and Solventogenic Anaerobes | 1 | 0 | |
| Encoding Genetic Circuits with DNA Barcodes Paves the Way for Machine Learning-Assisted Metabolite Biosensor Response Curve Profiling in Yeast | 1 | 1 | ⭐ |
| Uncovering the Distinct Properties of a Bacterial Type I-E CRISPR Activation System | 2 | 2 | ⭐ |
| GAMES: A Dynamic Model Development Workflow for Rigorous Characterization of Synthetic Genetic Systems | 1 | 0 |
March
| Article | No. of SBOL Visual compliant images | No. of SBOL Visual images following Best practices | |
|---|---|---|---|
| Synthetic Genetic Reversible Feynman Gate in a Single E. coli Cell and Its Application in Bacterial to Mammalian Cell Information Transfer | 2 | 1 | |
| Improving the Robustness of Engineered Bacteria to Nutrient Stress Using Programmed Proteolysis | 1 | 1 | ⭐ |
| Genome Engineering in Mycoplasma gallisepticum Using Exogenous Recombination Systems | 5 | 0 | |
| Conjugation-Based Genome Engineering in Deinococcus radiodurans | 3 | 0 | |
| Versatile Design of Intracellular Protein-Responsive Translational Regulation System for Synthetic mRNA | 4 | 0 | |
| A Dual-Purpose Real-Time Indicator and Transcriptional Integrator for Calcium Detection in Living Cells | 1 | 0 | |
| Design of Synthetic Mammalian Promoters Using Highly Palindromic Subsequences | 1 | 0 | |
| Engineering Transcription Factor XylS for Sensing Phthalic Acid and Terephthalic Acid: An Application for Enzyme Evolution | 1 | 1 | ⭐ |
| Systematic In Vivo Characterization of Fluorescent Protein Maturation in Budding Yeast | 1 | 0 | |
| Precise Thermal Regulation of Engineered Bacteria Secretion for Breast Cancer Treatment In Vivo | 2 | 0 | |
| Heterologous Synthesis and Secretion of Ricinoleic Acid in Starmerella bombicola with Sophorolipid as an Intermediate | 1 | 1 | ⭐ |
| Cre/lox-Mediated Chromosomal Integration of Biosynthetic Gene Clusters for Heterologous Expression in Aspergillus nidulans | 2 | 2 | ⭐ |
| Metabolic Division in an Escherichia coli Coculture System for Efficient Production of Kaempferide | 5 | 5 | ⭐ |
| Programmable Synthetic Upstream Activating Sequence Library for Fine-Tuning Gene Expression Levels in Saccharomyces cerevisiae | 2 | 2 | ⭐ |
| RNA Compensation: A Positive Feedback Insulation Strategy for RNA-Based Transcription Networks | 4 | 0 | |
| PASIV: A Pooled Approach-Based Workflow to Overcome Toxicity-Induced Design of Experiments Failures and Inefficiencies | 3 | 3 | ⭐ |
| Polymer Encapsulation of Bacterial Biosensors Enables Coculture with Mammalian Cells | 1 | 1 | ⭐ |
| Transcriptome Analysis of Bacillus licheniformis for Improving Bacitracin Production | 1 | 1 | ⭐ |
| RoboMoClo: A Robotics-Assisted Modular Cloning Framework for Multiple Gene Assembly in Biofoundry | 6 | 6 | ⭐ |
April
| Article | No. of SBOL Visual compliant images | No. of SBOL Visual images following Best practices | |
|---|---|---|---|
| Designing Biological Circuits: From Principles to Applications | 1 | 1 | ⭐ |
| Bioorthogonal Chemical Epigenetic Modifiers Enable Dose-Dependent CRISPR Targeted Gene Activation in Mammalian Cells | 2 | 1 | |
| Parameterized Computational Framework for the Description and Design of Genetic Circuits of Morphogenesis Based on Contact-Dependent Signaling and Changes in Cell–Cell Adhesion | 5 | 1 | |
| Phage Enzyme-Assisted Direct In Vivo DNA Assembly in Multiple Microorganisms | 1 | 1 | ⭐ |
| A CRISPR–Cas9 System-Mediated Genetic Disruption and Multi-fragment Assembly in Starmerella bombicola | 5 | 5 | ⭐ |
| DNA Input Classification by a Riboregulator-Based Cell-Free Perceptron | 4 | 0 | |
| [NiFe] Hydrogenase Accessory Proteins HypB–HypC Accelerate Proton Conversion to Enhance the Acid Resistance and d-Lactic Acid Production of Escherichia coli | 1 | 0 | |
| Construction and Application of a High-Throughput In Vivo Screening Platform for the Evolution of Nitrile Metabolism-Related Enzymes Based on a Desensitized Repressive Biosensor | 5 | 0 | |
| Genomic Iterative Replacements of Large Synthetic DNA Fragments in Corynebacterium glutamicum | 3 | 0 | |
| De Novo Production of Plant 4′-Deoxyflavones Baicalein and Oroxylin A from Ethanol in Crabtree-Negative Yeast | 1 | 1 | ⭐ |
| A Versatile Transcription Factor Biosensor System Responsive to Multiple Aromatic and Indole Inducers | 2 | 2 | ⭐ |
May
| Article | No. of SBOL Visual compliant images | No. of SBOL Visual images following Best practices | |
|---|---|---|---|
| Synthetic Translational Regulation by Protein-Binding RNA Origami Scaffolds | 4 | 4 | ⭐ |
| DeepTESR: A Deep Learning Framework to Predict the Degree of Translational Elongation Short Ramp for Gene Expression Control | 1 | 1 | ⭐ |
| Transcriptional Interference in Toehold Switch-Based RNA Circuits | 4 | 4 | ⭐ |
| Simulation-Based Engineering of Time-Delayed Safety Switches for Safer Gene Therapies | 2 | 2 | ⭐ |
| Increased Production of Riboflavin by Coordinated Expression of Multiple Genes in Operons in Bacillus subtilis | 3 | 0 | |
| Exploration of Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase Activity in Yeast | 1 | 0 | |
| Tuning Geraniol Biosynthesis via a Novel Decane-Responsive Promoter in Candida glycerinogenes | 1 | 0 | |
| Engineering Antisense Oligonucleotides as Antibacterial Agents That Target FMN Riboswitches and Inhibit the Growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli | 1 | 0 | |
| Combining CRISPR–Cpf1 and Recombineering Facilitates Fast and Efficient Genome Editing in Escherichia coli | 3 | 3 | ⭐ |
| Rerouting Fluxes of the Central Carbon Metabolism and Relieving Mechanism-Based Inactivation of l-Aspartate-α-decarboxylase for Fermentative Production of β-Alanine in Escherichia coli | 1 | 0 | |
| LOICA: Integrating Models with Data for Genetic Network Design Automation | 2 | 2 | ⭐ |
June
| Article | No. of SBOL Visual compliant images | No. of SBOL Visual images following Best practices | |
|---|---|---|---|
| Transcription-Associated Fluorescence-Activated Droplet Sorting for Di-rhamnolipid Hyperproducers | 1 | 1 | ⭐ |
| Temporal Expression of Transcription Factor ID2 Improves Natural Killer Cell Differentiation from Human Pluripotent Stem Cells | 1 | 0 | |
| Characterization of ColE1 Production for Robust tolC Plate Dual-Selection in E. coli | 1 | 0 | |
| Cell-Free Mutant Analysis Combined with Structure Prediction of a Lasso Peptide Biosynthetic Protease B2 | 1 | 1 | ⭐ |
| Efficient Synthesis of Phycocyanobilin by Combinatorial Metabolic Engineering in Escherichia coli | 1 | 0 | |
| High-Throughput Regulatory Part Prototyping and Analysis by Cell-Free Protein Synthesis and Droplet Microfluidics | 3 | 1 | |
| Efficient Single-Nucleotide Microbial Genome Editing Achieved Using CRISPR/Cpf1 with Maximally 3′-End-Truncated crRNAs | 1 | 1 | ⭐ |
| Dynamic Control Strategy to Produce Riboflavin with Lignocellulose Hydrolysate in the Thermophile Geobacillus thermoglucosidasius | 2 | 2 | ⭐ |
July
| Article | No. of SBOL Visual compliant images | No. of SBOL Visual images following Best practices | |
|---|---|---|---|
| The Growth Dependent Design Constraints of Transcription-Factor-Based Metabolite Biosensors | 5 | 4 | |
| Optimal LentiCRISPR-Based System for Sequential CRISPR/Cas9 Screens | 1 | 0 | |
| Engineering a Synthetic Dopamine-Responsive Riboswitch for In Vitro Biosensing | 1 | 0 | |
| High-Throughput Aminoacyl-tRNA Synthetase Engineering for Genetic Code Expansion in Yeast | 1 | 0 | |
| Control-Based Continuation: A New Approach to Prototype Synthetic Gene Networks | 1 | 1 | ⭐ |
| Machine Learning Guided Batched Design of a Bacterial Ribosome Binding Site | 1 | 1 | ⭐ |
| Light-Induced Patterning of Electroactive Bacterial Biofilms | 1 | 1 | ⭐ |
| Mitigating Host Burden of Genetic Circuits by Engineering Autonegatively Regulated Parts and Improving Functional Prediction | 5 | 5 | ⭐ |
| Tunable Temperature-Sensitive Transcriptional Activation Based on Lambda Repressor | 2 | 2 | ⭐ |
| pySBOL3: SBOL3 for Python Programmers | 1 | 1 | ⭐ |
August
| Article | No. of SBOL Visual compliant images | No. of SBOL Visual images following Best practices | |
|---|---|---|---|
| Standardization of Synthetic Biology Tools and Assembly Methods for Saccharomyces cerevisiae and Emerging Yeast Species | 6 | 5 | |
| PeroxiHUB: A Modular Cell-Free Biosensing Platform Using H2O2 as Signal Integrator | 2 | 0 | |
| A Modified Yeast Two-Hybrid Platform Enables Dynamic Control of Expression Intensities to Unmask Properties of Protein–Protein Interactions | 1 | 0 | |
| Second-Generation Escherichia coli SuptoxR Strains for High-Level Recombinant Membrane Protein Production | 1 | 0 | |
| Decoupling Growth and Production by Removing the Origin of Replication from a Bacterial Chromosome | 1 | 0 | |
| Implementation of a Novel Optogenetic Tool in Mammalian Cells Based on a Split T7 RNA Polymerase | 5 | 0 | |
| Engineering Agrobacterium tumefaciens Adhesion to Target Cells | 1 | 0 | |
| Engineering Critical Amino Acid Residues of Lanosterol Synthase to Improve the Production of Triterpenoids in Saccharomyces cerevisiae | 1 | 0 | |
| Whole-Cell Biosensor and Producer Co-cultivation-Based Microfludic Platform for Screening Saccharopolyspora erythraea with Hyper Erythromycin Production | 1 | 0 | |
| Rapid Assembly and Prototyping of Biocatalytic Virus-like Particle Nanoreactors | 3 | 0 | |
| Deciphering the Rules of Ribosome Binding Site Differentiation in Context Dependence | 2 | 0 | |
| Building the Plant SynBio Toolbox through Combinatorial Analysis of DNA Regulatory Elements | 2 | 0 | |
| Proteolytically Activated CRAC Effectors through Designed Intramolecular Inhibition | 3 | 0 | |
| Synergistic Improvement of 5-Aminolevulinic Acid Production with Synthetic Scaffolds and System Pathway Engineering | 1 | 0 | |
| Transfer RNA Synthesis-Coupled Translation and DNA Replication in a Reconstituted Transcription/Translation System | 2 | 0 | |
| Integrating CRISPR-Cas12a with a crRNA-Mediated Catalytic Network for the Development of a Modular and Sensitive Aptasensor | 2 | 2 | ⭐ |
| Stress-Tolerant, Recyclable, and Renewable Biocatalyst Platform Enabled by Engineered Bacterial Spores | 4 | 4 | ⭐ |
| Engineering Metabolic Pathways for Cofactor Self-Sufficiency and Serotonin Production in Escherichia coli | 4 | 4 | ⭐ |
| BMSS2: A Unified Database-Driven Modeling Tool for Systematic Biomodel Selection | 1 | 0 |
September
| Article | No. of SBOL Visual compliant images | No. of SBOL Visual images following Best practices | |
|---|---|---|---|
| Overcoming Leak Sensitivity in CRISPRi Circuits Using Antisense RNA Sequestration and Regulatory Feedback | 4 | 4 | ⭐ |
| Malarial Antibody Detection with an Engineered Yeast Agglutination Assay | 1 | 1 | ⭐ |
| Genome Editing by CRISPR/Cas12 Recognizing AT-Rich PAMs in Shewanella oneidensis MR-1 | 4 | 4 | ⭐ |
| Mobile Translation Systems Generate Genomically Engineered Escherichia coli Cells with Improved Growth Phenotypes | 1 | 0 | |
| An Easy-to-Use Plasmid Toolset for Efficient Generation and Benchmarking of Synthetic Small RNAs in Bacteria | 2 | 0 | |
| Prebiotics-Controlled Disposable Engineered Bacteria for Intestinal Diseases | 5 | 2 | |
| Evolution-Guided Biosynthesis of Terpenoid Inhibitors | 2 | 0 | |
| Nitrogenase Cofactor Maturase NifB Isolated from Transgenic Rice is Active in FeMo-co Synthesis | 1 | 0 | |
| GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for Fine-Tuned Recombinant Gene Expression in Plants | 4 | 0 | |
| CRISPR/Cpf1-Mediated Multiplex and Large-Fragment Gene Editing in Staphylococcus aureus | 5 | 2 | |
| A Network Approach to Genetic Circuit Designs | 3 | 2 | |
| Modular Use of the Uniquely Small Ring A of Mersacidin Generates the Smallest Ribosomally Produced Lanthipeptide | 1 | 0 | |
| Enzymatic Supercoiling of Bacterial Chromosomes Facilitates Genome Manipulation | 1 | 0 | |
| Inducible CRISPR/Cas9 Allows for Multiplexed and Rapidly Segregated Single-Target Genome Editing in Synechocystis Sp. PCC 6803 | 7 | 1 | |
| A Universal Method for Developing Autoinduction Expression Systems Using AHL-Mediated Quorum-Sensing Circuits | 2 | 2 | ⭐ |
October
| Article | No. of SBOL Visual compliant images | No. of SBOL Visual images following Best practices | |
|---|---|---|---|
| Intestinal Engineered Probiotics as Living Therapeutics: Chassis Selection, Colonization Enhancement, Gene Circuit Design, and Biocontainment | 4 | 1 | |
| Interaction between the Anchoring Domain of A-Kinase Anchoring Proteins and the Dimerization and Docking Domain of Protein Kinase A: A Potent Tool for Synthetic Biology | 1 | 0 | |
| Synonymous Codons and Hydrophobicity Optimization of Post-translational Signal Peptide PelB Increase Phage Display Efficiency of DARPins | 1 | 0 | |
| A p-Coumaroyl-CoA Biosensor for Dynamic Regulation of Naringenin Biosynthesis in Saccharomyces cerevisiae | 2 | 0 | |
| A Versatile in Vivo DNA Assembly Toolbox for Fungal Strain Engineering | 1 | 1 | ⭐ |
| Steady-State Operation of a Cell-Free Genetic Band-Detection Circuit | 2 | 2 | ⭐ |
| Construction of an rAAV Producer Cell Line through Synthetic Biology | 1 | 0 | |
| Novel Split Intein-Mediated Enzymatic Channeling Accelerates the Multimeric Bioconversion Pathway of Ginsenoside | 2 | 2 | ⭐ |
| Reprogramming the Cleavage Specificity of Botulinum Neurotoxin Serotype B1 | 3 | 0 | |
| SpyPhage: A Cell-Free TXTL Platform for Rapid Engineering of Targeted Phage Therapies | 2 | 1 | |
| Optogenetic Control of Bacterial Expression by Red Light | 1 | 0 | |
| Induced CD45 Proximity Potentiates Natural Killer Cell Receptor Antagonism | 2 | 0 | |
| Efficient Bacterial Genome Engineering throughout the Central Dogma Using the Dual-Selection Marker tetAOPT | 5 | 1 | |
| Reprogramming T7RNA Polymerase in Escherichia coli Nissle 1917 under Specific Lac Operon for Efficient p-Coumaric Acid Production | 3 | 0 | |
| Light-Dependent Control of Bacterial Expression at the mRNA Level | 3 | 2 | |
| Discovery and Heterologous Expression of Microginins from Microcystis aeruginosa LEGE 91341 | 3 | 3 | ⭐ |
| Engineered Protein Nanocages for Concurrent RNA and Protein Packaging In Vivo | 2 | 1 | |
| Building an Artificial Plant Cell Wall on a Lipid Bilayer by Assembling Polysaccharides and Engineered Proteins | 1 | 0 | |
| Analysis of Slow-Cycling Variants of the Light-Inducible Nuclear Protein Export System LEXY in Mammalian Cells | 1 | 0 | |
| GroovDB: A Database of Ligand-Inducible Transcription Factors | 2 | 0 | |
| The VEGAS Platform Is Unsuitable for Mammalian Directed Evolution | 2 | 1 |
November
| Article | No. of SBOL Visual compliant images | No. of SBOL Visual images following Best practices | |
|---|---|---|---|
| Engineered Spore-Forming Bacillus as a Microbial Vessel for Long-Term DNA Data Storage | 2 | 0 | |
| Harnessing Escherichia coli’s Native Machinery for Detection of Vitamin C (Ascorbate) Deficiency | 2 | 0 | |
| Unusual Class I Lanthipeptides from the Marine Bacteria Thalassomonas viridans | 3 | 2 | |
| Synthetic Biology Toolbox, Including a Single-Plasmid CRISPR-Cas9 System to Biologically Engineer the Electrogenic, Metal-Resistant Bacterium Cupriavidus metallidurans CH34 | 2 | 1 | |
| Genome-Wide Screen for Enhanced Noncanonical Amino Acid Incorporation in Yeast | 2 | 0 | |
| AgeMTPT, a Catalyst for Peptide N-Terminal Modification | 1 | 1 | ⭐ |
| Genetic Code Expansion in Pseudomonas putida KT2440 | 2 | 2 | ⭐ |
| Synthetic Platforms for Characterizing and Targeting of SARS-CoV-2 Genome Capping Enzymes | 4 | 4 | ⭐ |
| MGCEP 1.0: A Genetic-Engineered Marine-Derived Chassis Cell for a Scaled Heterologous Expression Platform of Microbial Bioactive Metabolites | 3 | 1 | |
| Comparison of Kill Switch Toxins in Plant-Beneficial Pseudomonas fluorescens Reveals Drivers of Lethality, Stability, and Escape | 3 | 1 | |
| Engineering a Rhodopsin-Based Photo-Electrosynthetic System in Bacteria for CO2 Fixation | 1 | 0 | |
| Zymo-Parts: A Golden Gate Modular Cloning Toolbox for Heterologous Gene Expression in Zymomonas mobilis | 1 | 0 | |
| EasyGuide Plasmids Support in Vivo Assembly of gRNAs for CRISPR/Cas9 Applications in Saccharomyces cerevisiae | 1 | 0 |
December
| Article | No. of SBOL Visual compliant images | No. of SBOL Visual images following Best practices | |
|---|---|---|---|
| Ribosome Stalling of N-Linked Glycoproteins in Cell-Free Extracts | 1 | 1 | ⭐ |
| Robotic Construction and Screening of Lanthipeptide Variant Libraries in Escherichia coli | 2 | 2 | ⭐ |
| Targeting Transcriptional and Translational Hindrances in a Modular T7RNAP Expression System in Engineered Pseudomonas putida | 8 | 0 | |
| Two-Layered Microfluidic Devices for High-Throughput Dynamic Analysis of Synthetic Gene Circuits in E. coli | 3 | 3 | ⭐ |
| High Titer of (S)-Equol Synthesis from Daidzein in Escherichia coli | 3 | 0 | |
| Tuning of Gene Expression in Clostridium phytofermentans Using Synthetic Promoters and CRISPRi | 4 | 0 | |
| Hybrid Nucleic Acid-Quantum Dot Assemblies as Multiplexed Reporter Platforms for Cell-Free Transcription Translation-Based Biosensors | 1 | 1 | ⭐ |
| Rapid and Accurate Assembly of Large DNA Assisted by In Vitro Packaging of Bacteriophage | 6 | 3 | |
| Computer-Based Design of a Cell Factory for High-Yield Cytidine Production | 1 | 1 | ⭐ |
| Enzymatic Synthesis of DNA with an Expanded Genetic Alphabet Using Terminal Deoxynucleotidyl Transferase | 1 | 1 | ⭐ |
| Modulating DHA-Producing Schizochytrium sp. toward Astaxanthin Biosynthesis via a Seamless Genome Editing System | 4 | 3 | |
| Characterization of Eight Bacterial Biosensors for Microbial Diagnostic and Therapeutic Applications | 1 | 0 | |
| Autoinduction Expression Modules for Regulating Gene Expression in Bacillus subtilis | 2 | 2 | ⭐ |
